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@PHDTHESIS{Goldmanns:1004264,
author = {Goldmanns, Jennifer},
othercontributors = {Lauterbach, Lars and Büchs, Jochen},
title = {{H}igh-throughput online monitoring techniques for
bioprocesses with spore-forming bacteria},
school = {RWTH Aachen University},
type = {Dissertation},
address = {Aachen},
publisher = {RWTH Aachen University},
reportid = {RWTH-2025-01323},
pages = {1 Online-Ressource : Illustrationen},
year = {2024},
note = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
University 2025; Dissertation, RWTH Aachen University, 2024},
abstract = {The optimization of cultivation parameters is crucial for
efficient microbial processes. Therefore, shaken
high-throughput cultivation systems combined with online
monitoring techniques are required in early bioprocess
development. In this thesis, the potential of online
monitoring techniques to evaluate growth and sporulation in
cultivations of spore-forming bacteria was investigated. A
key parameter for achieving high cell densities is a
tailor-made cultivation medium that complies with the
specific nutrient requirements of the respective
microorganism. A chemically defined medium was developed,
exemplarily, for an industrial Paenibacillus polymyxa strain
in microtiter plate scale. For this purpose, a method,
combining a systematic experimental procedure with online
monitoring of the respiration activity, was used. The method
was extended to identify nutrient limitations. After
improving the growth, the medium components were reduced to
the growth-relevant ones. The cultivation with the final
medium was transferred from the microtiter plate to the
laboratory fermenter scale. In addition to high cell
densities, the characterization of sporulation is relevant
to achieve high spore concentrations in bioprocesses with
spore-forming bacteria. However, conventional spore
determination methods are laborious. Therefore,
spectroscopic techniques were applied in this thesis for
online monitoring of Bacillus subtilis sporulation in
microtiter plate cultivations with complex medium. A
specific fluorescence wavelength was identified. The
intensity of this fluorescence wavelength correlated with
the release of spores from the mother cells. Strong
indications demonstrated that siderophores cause this
characteristic fluorescence. The universality of the
fluorescence measurement for spore detection was validated
with other Bacillus species. In addition, the method was
used to investigate the influence of the CaCl2 concentration
and oxygen availability on the sporulation of B. subtilis.
Finally, the applicability of the online spore detection
method for a chemically defined medium was shown. The
results presented in this work provide the basis for the
future usage of high-throughput online measurement
techniques to optimize cultivations of spore-forming
bacteria in a reliable and time-efficient manner.},
cin = {161820 / 160000 / 416510},
ddc = {570},
cid = {$I:(DE-82)161820_20140620$ / $I:(DE-82)160000_20140620$ /
$I:(DE-82)416510_20140620$},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2025-01323},
url = {https://publications.rwth-aachen.de/record/1004264},
}
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