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%0 Thesis
%A Voß, Leonie Fidelis Johanna Margarete
%T Hochskalierung der Herstellung eines zellfreien Proteinsynthesesystems aus Tabakzellen und Entwicklung eines Verfahrens zur Durchführung der Reaktion im Liter-Maßstab
%I RWTH Aachen University
%V Dissertation
%C Aachen
%M RWTH-2025-01489
%P 1 Online-Ressource : Illustrationen
%D 2025
%Z Veröffentlicht auf dem Publikationsserver der RWTH Aachen University
%Z Dissertation, RWTH Aachen University, 2025
%X In this work, an upscaling of the lysate preparation and the CFPS reaction of Nicotiana tabacum cv. BY-2 cell lysate was investigated to enable the production of difficult-to-produce proteins in larger protein quantities. For the preparation of lysate with a final volume of 1 L, important preparation steps such as the cultivation and protoplast formation of the BY-2 cells were transferred from the shake flask process to a stirred tank reactor (STR) process. The transfer of the cultivation process for the purpose of lysate production was successful, but with up to 30 
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%R 10.18154/RWTH-2025-01489
%U https://publications.rwth-aachen.de/record/1004548