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%0 Thesis
%A Srdic, Matic
%T Development and application of the human cytochrome P450 monooxygenase 3A4 for preparative scale biocatalysis of valuable pharmaceutical compounds
%I RWTH Aachen University
%V Dissertation
%C Aachen
%M RWTH-2025-02940
%P 1 Online-Ressource : Illustrationen
%D 2024
%Z Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2025
%Z Dissertation, RWTH Aachen University, 2024
%X Cytochromes P450 (CYPs) are a vital group of enzymes found in all the kingdoms of life where they primarily function as monooxygenases, incorporating hydroxyl groups into a variety of substrates to detoxify xenobiotics or as part of a synthetic pathway. In humans they are best known for metabolizing drugs or other xenobiotics and synthesizing essential compounds like steroid hormones. Their genetic diversity and unique oxygenation capability make them interesting targets for biotechnological research. Here they are particularly prized for both the wide variety of accepted substrates and capability for exceptional regio- and stereoselectivity in production of valuable compounds such as drugs or fine chemicals. As important mediators of drug metabolism and clearance they are routinely utilized in metabolite identification (MetID) studies and metabolite in safety testing (MIST). One of the reasons for the high cost of bringing a drug to market is the high attrition rate of potential drug candidates due to drug safety failures or its inability to be effective at safe doses. Developing a novel biocatalyst based on the human CYP3A4, the enzyme involved in the metabolism of over 50
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%R 10.18154/RWTH-2025-02940
%U https://publications.rwth-aachen.de/record/1008313