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TY  - THES
AU  - Al Asmi, Mohammad Nour
TI  - Aufklärung des Effekts von Nrf2 auf parodontale Erkrankungen in vitro
PB  - Rheinisch-Westfälische Technische Hochschule Aachen
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2025-04752
SP  - VI, 74 Seiten : Illustrationen
PY  - 2025
N1  - Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2025
AB  - Periodontal diseases (PD) are among the most significant health issues worldwide. They are caused by bacterial infections, including Porphyromonas gingivalis (P. g.), which can damage the periodontal ligament and alveolar bone, leading to tooth loss and a significant reduction in quality of life. Lipopolysaccharide (LPS) from P. g. is considered a critical factor in the onset and development of PD, as it induces the formation of intracellular reactive oxygen species (ROS). The accumulation of ROS leads to oxidative stress, influencing the progression of PD and degrading nuclear factor erythroid-derived 2-related factor 2 (Nrf2), which plays a crucial role in bone healing. This study investigates the impact of LPS-P. g. as an ROS inducer and methysticin as an Nrf2 activator on the proliferation and mineralization of preosteoblasts from the MC3T3-E1 (subclone 4) cell line. Cells were cultured in α-MEM and stimulated with LPS-P. g. (STD and ULT). NF-κB activity was determined using a dual luciferase assay, while cell proliferation and cytotoxicity were assessed using the CyQUANT® Cell Assay. Mineralization was evaluated by Alizarin Red staining of calcium deposits after a 28-day differentiation process, and ALP activity was measured. The protein concentration of IL-6, IL-1ß, VEGF, and TNF-α was determined using sandwich ELISAs. Results showed weak activation of NF-κB in MC3T3-E1 cells compared to RAW 264.7 macrophages after stimulation with 1µg/ml LPS-P. g. (STD). LPS-P. g. (STD and ULT) had no inhibitory effect on cell proliferation. However, LPS P. g. (STD) could inhibit the differentiation of MC3T3-E1 cells and their ALP activity. Conversely, methysticin positively affected ALP activity and mineralization. Injection of LPS-P. g. (STD) increased IL-6 expression and decreased VEGF expression. In summary, the concentration of 1µg/ml LPS-P. g. (STD) used is significantly above the physiological range and could cause decreased mineralization of MC3T3-E1 cells due to inflammation or increased ROS formation. Methysticin, due to its antioxidative properties, could potentially be developed as a drug for the treatment or prevention of PD in the future. However, its toxicity needs to be considered in the overall assessment.
LB  - PUB:(DE-HGF)11
UR  - https://publications.rwth-aachen.de/record/1011826
ER  -