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@PHDTHESIS{Mohapatra:1012085,
      author       = {Mohapatra, Saurav Ranjan},
      othercontributors = {Apel, Christian and Kiessling, Fabian},
      title        = {{D}evelopment and non-invasive monitoring of
                      tissue-engineered cardiovascular implants},
      school       = {Rheinisch-Westfälische Technische Hochschule Aachen},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2025-04854},
      pages        = {1 Online-Ressource : Illustrationen},
      year         = {2025},
      note         = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
                      University; Dissertation, Rheinisch-Westfälische Technische
                      Hochschule Aachen, 2025, Kumulative Dissertation},
      abstract     = {Cardiovascular diseases (CVD) are among the leading causes
                      of death globally, requiring advancements in treatment
                      options. Synthetic vascular grafts and mechanical heart
                      valves have limitations such as thrombosis and the need for
                      repeated surgeries. Tissue-engineered vascular grafts
                      (TEVGs) and heart valves (TEHVs) offer promising
                      alternatives due to their remodeling capabilities and
                      potential to integrate with host tissues. However,
                      challenges remain in ensuring mechanical strength, rapid
                      extracellular matrix (ECM) maturation, and real-time
                      monitoring. Additionally, effective implant life cycle
                      management and long-term follow-up are critical for clinical
                      success. Our research aims to accelerate the development of
                      TEVGs and TEHVs through advanced bioreactor conditioning and
                      7T MRI monitoring, bringing them closer to clinical
                      application.The first study aimed to enhance the production
                      of TEVGs by significantly shortening the bioreactor
                      conditioning time, which normally takes several weeks. A
                      biodegradable polylactic-co-glycolic acid (PLGA) scaffold,
                      combined with a copolymer-reinforced fibrin gel, was used as
                      a cell carrier. By utilizing the combination of textile,
                      biodegradable fiber, and hydrogel matrix, the conditioning
                      time was reduced to just 4 days, while achieving substantial
                      extracellular matrix (ECM) maturation and mechanical
                      strength. Mechanical testing showed the TEVG’s burst
                      pressure (617±85 mm Hg) and tensile strength exceeded that
                      of native arteries. Additionally, the incorporation of MRI
                      contrast agents into the scaffold allowed for non-invasive
                      in vitro monitoring of scaffold degradation using 7T MRI,
                      providing valuable insights into the remodeling process. The
                      second study focused on the development of a pneumatically
                      driven multimodal bioreactor optimized for MRI and
                      ultrasound imaging for tissue-engineered heart valves
                      (TEHVs). A custom-built bioreactor, designed to operate
                      dynamically within a 72 mm bore of a 7T MRI coil, enabled
                      the real-time monitoring of TEHV functionality without
                      causing MRI interference. TEHVs were constructed using a
                      polyethylene terephthalate (PET) scaffold and human arterial
                      cells, conditioned under physiological conditions. The
                      bioreactor system facilitated the production of ECM
                      components, including collagen and smooth muscle actin,
                      while ensuring a monolayer of endothelial cells. MRI motion
                      compensation techniques and ultrasound imaging successfully
                      captured the dynamic movement of the heart valves, further
                      proving the compatibility of this novel system for
                      longitudinal monitoring.In conclusion, these two studies
                      underscore the potential of tissue-engineered cardiovascular
                      implants for future clinical use. By drastically reducing
                      the conditioning time of TEVGs and enabling dynamic
                      real-time imaging of TEHVs, we have moved closer to
                      developing implants that are not only functional and durable
                      but also capable of being non-invasively monitored during
                      their development and post-implantation phases. These
                      advancements represent a significant step toward creating
                      cardiovascular implants that can be efficiently translated
                      to clinical practice, improving patient outcomes and
                      reducing the need for repeated interventions.},
      cin          = {811001-1 ; 923510},
      ddc          = {610},
      cid          = {$I:(DE-82)811001-1_20140620$},
      pnm          = {DFG project G:(GEPRIS)403039938 - TexValveMonitoring -
                      Multimodale longitudinale Bildgebung von biohybriden
                      Herzklappen (403039938)},
      pid          = {G:(GEPRIS)403039938},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2025-04854},
      url          = {https://publications.rwth-aachen.de/record/1012085},
}