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@PHDTHESIS{Wang:1012468,
author = {Wang, Jiayun},
othercontributors = {Walter, Peter and Weis, Joachim},
title = {{A} workflow to visualize vertebrate eyes in 3{D}},
school = {Rheinisch-Westfälische Technische Hochschule Aachen},
type = {Dissertation},
address = {Aachen},
publisher = {RWTH Aachen University},
reportid = {RWTH-2025-05010},
pages = {1 Online-Ressource : Illustrationen},
year = {2025},
note = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
University; Dissertation, Rheinisch-Westfälische Technische
Hochschule Aachen, 2025},
abstract = {Purpose:To establish a workflow to visualize the surgical
anatomy in 3D based on histological data of eyes of
experimental animals for improving the planning of complex
surgical procedures.Methods: Four C57BL/6J wild-type(wt)
mouse eyes, three Brown Norway rat eyes and four Chinchilla
Bastard rabbit eyes were enucleated and processed for
standard histology with serial sections and hematoxylin and
eosin staining. Image stacks were processed to obtain a
representation of the eye anatomy in 3D. In addition,
virtual image stacks and 3D point clouds were generated by
processing sagittal sections of eyes with stepwise 180°
rotation and projection around the eye axis to construct a
rotationally symmetric 3D model from one single sagittal
section.ResultsSerial sections of whole eyes of mice, rats
and rabbits showed significant artifacts interfering with a
practical image stack generation and straightforward 3D
reconstruction despite the application of image registration
techniques. A workflow was established to obtain a 3D image
of the eye based on virtual image stacks and point cloud
generation by rotation of a single sagittal section of the
eye around the symmetry axis. By analyzing the tissue
shrinkage during histological processing true biometric
reconstructions of the eyes were feasible making the
resulting model usable for 3D modeling and simulation, e.g.
for planning of complex surgical procedures in different
species.Conclusion: Because serial sections of the eye with
standard histological protocols yielded too many artifacts
for a straightforward 3D visualization, we reconstructed a
pseudorealistic 3D model based on virtual image stacks and
point cloud generation calculated from a single sagittal
section of the eye. Such a model detailing microscopic
structures of the whole eye will allow for a specific
planning of surgical procedures in small animal eyes in
order to prevent surgical complications in a very early
stage of an experiment and it will support the design and
development of complex intraocular implants. It will
therefore be helpful in surgical teaching and improve
laboratory animal welfare by an expected reduction of
experimental animal numbers. Further processing including
integration of mechanical tissue properties is needed to
convert these 3D models into a practical virtual reality
teaching and simulation platform for eyes of several
species.},
cin = {536000-2 ; 938110},
ddc = {610},
cid = {$I:(DE-82)536000-2_20140620$},
pnm = {GRK 2610 - GRK 2610: Innovative Schnittstellen zur Retina
für optimiertes künstliches Sehen - InnoRetVision
(424556709)},
pid = {G:(GEPRIS)424556709},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2025-05010},
url = {https://publications.rwth-aachen.de/record/1012468},
}
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