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%0 Thesis
%A Cheng, Jianan
%T Zinc supplementation reduces the formation of neutrophil extracellular traps by decreasing the expression of peptidyl arginine deiminase 4
%I Rheinisch-Westfälische Technische Hochschule Aachen
%V Dissertation
%C Aachen
%M RWTH-2025-07841
%P 11 Blätter : Illustrationen
%D 2025
%Z Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2025
%X Neutrophils play a decisive role during the immediate defense against infections. However, their deregulation, as observed during rheumatoid arthritis, can also cause tissue damage. Previous studies indicate that zinc supplementation may alter certain neutrophil functions. However, the precise underlying mechanisms as well as possible effects of zinc deficiency remain incompletely understood. The objective of this study was to investigate the effects of zinc homeostasis on formation of neutrophil extracellular traps (NETs) and other fundamental neutrophil functions. We used interleukin (IL)-17 and tumor necrosis factor (TNF)-α to simulate the inflammatory environment observed in autoimmune diseases. Subsequently, we analyzed the impact of different extracellular zinc concentrations on the release of NETs, using a fluorescence plate reader, as well as on the expression of peptidylarginine deiminase 4 (PAD4), S100A8/A9 and certain cytokines by PCR and western blot. Our research shows that zinc supplementation significantly reduces NETs formation, which may involve downregulation PAD4 expression. Additionally, zinc supplementation resulted in increasing the expression of interleukin-1 receptor antagonist (IL-1RA) and IL-8. The results demonstrate that changes in extracellular zinc availability can influence the functions of neutrophils. Therefore, maintaining an appropriate zinc level is crucial for preserving the function of innate immunity.
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%U https://publications.rwth-aachen.de/record/1018423