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@PHDTHESIS{Hansen:1020439,
author = {Hansen, Nadja},
othercontributors = {Fischer, Horst and Jockenhövel, Stefan},
title = {{E}ntwicklung eines 3{D}-biogedruckten
{B}lutgefäßersatzes zur {U}ntersuchung des zellulären
{V}erhaltens in {I}n-vitro-{G}ewebemodellen unter
dynamischer {K}ultivierung},
school = {Rheinisch-Westfälische Technische Hochschule Aachen},
type = {Dissertation},
address = {Aachen},
publisher = {RWTH Aachen University},
reportid = {RWTH-2025-08995},
pages = {1 Online-Ressource : Illustrationen},
year = {2025},
note = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
University 2026; Dissertation, Rheinisch-Westfälische
Technische Hochschule Aachen, 2025},
abstract = {The present work describes the development of a novel
3D-bioprinted in vitro model of a blood vessel to provide
new insights into the mechanisms of tissue angiogenesis and
to test new drug substances. A bioreactor made of PEEK was
developed, which enables direct imaging and perfusion.
Furthermore, different collagen- and fibrin-based hydrogels
were tailored to the different cell types, compared with
each other and the cell-ECM interactions were studied. The
results show the benefit of pure 2.5 $\%$ fibrin for
obtaining a stable endothelial layer as a lining for the
3D-bioprinted macrovessels. In contrast, collagen-fibrin
compositions with larger pores and less stiffness were
particularly suitable for tissue models of the liver and as
matrices of tumor spheroids. In particular, hepatocytes
showed an increased metabolic activity by an increased
production of albumin in the hydrogel that corresponded to
the stiffness of the healthy liver (4.5 kPa). It was also
shown, that the introduction of fibroblast and HUVECs into
the matrix had a positive influence on cell adhesion and
metabolism. HUVECs in the surrounding matrix increased the
formation of microvessels from the printed macrovessel. It
was advantageous if the printed HUVECs were first cultured
in the gelatine for three hours to adhere to the surrounding
matrix, before the gel was rinsed out. The endothelial layer
was significantly more stable if it had 72 hours to form
under static conditions before the flow through and thus the
dynamic cultivation started. Using the double sacrificial
material method, branched vascular structures up to a
diameter of 10 µm could be produced. The combination of the
developed in vitro model with a further developed liver and
the tumor model could provide new insights into the
metastatic cascade and subsequently for new approaches for
cancer therapy.},
cin = {542000-2 ; 937710 / 520000},
ddc = {620},
cid = {$I:(DE-82)542000-2_20140620$ / $I:(DE-82)520000_20140620$},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2025-08995},
url = {https://publications.rwth-aachen.de/record/1020439},
}
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