32 Amels, Anna Maria Beier, Justus Eickhoff, Roman 533530-2 ; 937310 RWTH Aachen University Aachen German 1 Online-Ressource : Illustrationen Complex culture media are commonly used to direct the differentiation of mesenchymal stem cells in vitro. However, their composition presents a major challenge for clinical application, as many components carry potential in vivo risks. A key goal in tissue engineering is therefore to reduce potentially hazardous supplements while maintaining effective differentiation. This dissertation investigated the effects of calcium and magnesium ions on the osteogenic and myogenic differentiation of ASCs and HSKs. Under the experimental conditions applied in this work, osteogenesis could not be induced by these ions alone. Nevertheless, early osteogenic phases were enhanced, and cell proliferation increased, suggesting a synergistic interaction with the established differentiation medium. There were also indications of a pro-myogenic effect of the ions, which require further quantitative validation. Overall, the results confirm the biocompatibility of the applied compounds and highlight their potential for tissue engineering applications. Under the conditions used in this study, calcium and magnesium ions supported differentiation processes but could not fully replace established supplements. Veröffentlicht auf dem Publikationsserver der RWTH Aachen University ; Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2026 ; 2, ; PUB:(DE-HGF)11, ; Dissertation / PhD Thesis Dissertation Rheinisch-Westfälische Technische Hochschule Aachen 2026 2026 RWTH-2026-01837 2026 https://publications.rwth-aachen.de/record/1028721