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P4SB

P4SB – From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology

CoordinatorLeipzig University ; UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN ; BACMINE SL ; CNRS - Institut des Sciences Biologiques ; Helmholtz-Zentrum für Umweltforschung GmbH - UFZ ; PROTEUS ; SOPREMA ; AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS ; University of Surrey ; RWTH Aachen University ; BIOPLASTECH LTD
Grant period2015-04-01 - 2019-03-31
Funding bodyEuropean Union
Call numberH2020-LEIT-BIO-2014-1
Grant number633962
IdentifierG:(EU-Grant)633962

Note: P4SB is about the utilization of the conceptual and material tools of contemporary Synthetic Biology to bring about the sustainable and environmentally friendly bioconversion of oil-based plastic waste into fully biodegradable counterparts by means of deeply engineered, whole-cell bacterial catalysts. These tools will be used to design tailor-made enzymes for the bio-depolymerization of PET (polyethylene terephthalate) and PU (polyurethane), but also for the custom design of a Pseudomonas putida Cell Factory capable of metabolizing the resulting monomers. Pseudomonas putida will undergo deep metabolic surgery to channel these diverse substrates efficiently into the production of polyhydroxyalkanoates (PHA) and derivatives. In addition, synthetic downstream processing modules based on the programmed non-lytic secretion of PHA will facilitate the release and recovery of the bioplastic from the bacterial biomass. These industry driven objectives will help to address the market need for novel routes to valorise the gigantic plastic waste streams in the European Union and beyond, with direct opportunities for SME partners of P4SB spanning the entire value chain from plastic waste via Synthetic Biology to biodegradable plastic. As a result we anticipate a completely biobased process reducing the environmental impact of plastic waste by establishing it as a novel bulk second generation carbon source for industrial biotechnology, while at the same time opening new opportunities for the European plastic recycling industry and helping to achieve the ambitious recycling targets set by the European Union for 2020.
     

Recent Publications

All known publications ...
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http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Dissertation / PhD Thesis/Book  ;  ;
Development of synthetic biology tools for Pseudomonas putida
Aachen : Apprimus Verlag, Applied microbiology 23, : 1. Auflage, 1 Online-Ressource (XVI, 162 Seiten) : Illustrationen, Diagramme () [10.18154/RWTH-2020-09053] = Dissertation, RWTH Aachen University, 2020  GO OpenAccess  Download fulltext Files BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article (Letter)  ;  ;  ;  ;  ;  ;  ;  ;
An engineered PQQ-dependent alcohol dehydrogenase for the oxidation of 5-(hydroxymethyl)furoic acid
ACS catalysis 10(14), 7836-7842 () [10.1021/acscatal.0c01789]  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Journal Article  ;  ;
Characterization of Context-Dependent Effects on Synthetic Promoters
Frontiers in Bioengineering and Biotechnology 8, 551 () [10.3389/fbioe.2020.00551]  GO OpenAccess  Download fulltext Files BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Poster  ;  ;
Identification of reliable genomic landing sites for heterologous constructs inPseudomonas putida KT2440
2. Pseudomonas Grassroots Meeting, PGM-2, LeidenLeiden, Netherlands, 7 Nov 2019 - 8 Nov 20192019-11-072019-11-08  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Poster  ;  ;
Synthetic promoter stacking: when two plus two equals three
GASB - German Conference on Synthetic Biology, GCSB, AachenAachen, Germany, 12 Sep 2019 - 13 Sep 20192019-09-122019-09-13  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Conference Presentation (After Call)  ;  ;
Development and application of molecular tools for Pseudomonas
1. Pseudomonas Grassroots Meeting, Frankfurt am MainFrankfurt am Main, Germany, 8 Nov 2018 - 9 Nov 20182018-11-082018-11-09  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Conference Presentation (After Call)  ;  ;
Synthetic promoter stacking: When two plus two equals three
4. Meeting in Applied Synthetic Biology in Europe, ToulouseToulouse, France, 24 Oct 2018 - 26 Oct 20182018-10-242018-10-26  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Talk (non-conference) (Invited)  ;  ;
P4SB - From Plastic waste to Plastic value using Pseudomonas putida Synthetic Biology
Seminartag Bioplastik, MünsterMünster, Germany, 11 Jun 2016 - 11 Jun 20162016-06-112016-06-11  GO BibTeX | EndNote: XML, Text | RIS

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Conference Presentation  ;  ;  ;
Synthetic promoter libraries, a powerful tool for tuning gene expression
3. Congress on Applied Synthetic Biology in Europe, LisbonLisbon, Portugal, 22 Feb 2016 - 24 Feb 20162016-02-222016-02-24  GO BibTeX | EndNote: XML, Text | RIS

All known publications ...
Download: BibTeX | EndNote XML,  Text | RIS | 

 Record created 2015-09-17, last modified 2023-02-15
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