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@PHDTHESIS{Hsing:51199,
author = {Hüsing, Corinna Elisabeth Gesina},
othercontributors = {Korr, Hubert},
title = {{A}utoradiographische {M}odelluntersuchungen an der {M}aus
zum {E}influss einer achtwöchigen
50-{H}z-{M}agnetfeld-{E}xposition auf {DNA}-{R}eparatur und
{Z}ellproliferation in der {M}ilz},
address = {Aachen},
publisher = {Publikationsserver der RWTH Aachen University},
reportid = {RWTH-CONV-113511},
pages = {V, 49 S. : Ill., graph. Darst.},
year = {2009},
note = {Aachen, Techn. Hochsch., Diss., 2009},
abstract = {The aim of this thesis was to study autoradiographically
after injection of ³H-thymidine (TdR) the influence of an
8-week magnetic field (MF) exposure of 50 Hz with 0.1 mT or
1 mT flux density on cell proliferation in the spleen
germinal center and nuclear (n) DNA-repair capacity in the
mantle zone of follicular spleen cells of adult mice.
Altogether 72 male mice were exposed to a 50 Hz magnetic
field with flux densities of 0 mT (control), 0.1 mT or 1 mT
for a period of eight weeks. In addition, three completely
untreated animals were used for determination of
autoradiographic background labeling. The mice were injected
with ³H-TdR 5 minutes (sub-group A), 24 hours (sub-group B)
or seven days (sub-group C) after the end of MF exposure.
All mice were sacrificed 115 min after ³H-TdR injection
with an intracardial perfusion fixation. 3 µm thick
paraffin sections were deparaffinized, Feulgen stained, and
autoradiographs were prepared using Ilford K2-emulsion and
the dipping method. The exposition time was 251 days. In
order to study cell proliferation in the spleen germinal
center, the total number of follicles per section as well as
the number of follicles containing so-called clusters, i.e.
accumulations of S phase cells, and the number of S-phase
cells per 10 follicles were determined. The (relative)
extend of nDNA damage of spleen mantle zone cells was
calculated by measuring nuclear silver grain numbers. Using
cumulative frequency distributions of the silver grains,
artifacts (arisen during the production of the
autoradiographs) could be recognized; these autoradiographs
were excluded from further calculations. The collected data
were analyzed using univariance analysis (generalized linear
model) and afterwards subjected to post hoc t-tests
considering Bonferroni’s correction. For p <0.05 no
statistically significant results were found. This indicates
that a magnetic field exposure as used had no influence on
the cell proliferation in the germinal zone of the spleen
and did not lead to (measurable) nDNA damage.},
keywords = {Autoradiographie (SWD) / DNS-Reparatur (SWD) / Milzpulpa
(SWD) / Milz (SWD) / Milzkreislauf (SWD)},
cin = {511001-5},
ddc = {610},
cid = {$I:(DE-82)511001-5_20140620$},
typ = {PUB:(DE-HGF)11},
urn = {urn:nbn:de:hbz:82-opus-28668},
url = {https://publications.rwth-aachen.de/record/51199},
}
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