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@PHDTHESIS{Kruse:56523,
author = {Kruse, Cordula Maria},
othercontributors = {Slusarenko, Alan},
title = {{C}harakterisierung des {GR}1-{G}ens aus {A}rabidopsis
thaliana ({L}.) {H}eynh. im {H}inblick auf die
rassenspezifische {R}esistenz des Ökotypen {W}assilewskija
gegenüber dem {P}eronospora parasitica-{I}solat {WELA}},
address = {Aachen},
publisher = {Publikationsserver der RWTH Aachen University},
reportid = {RWTH-CONV-118618},
pages = {143 S. : Ill., graph. Darst.},
year = {2001},
note = {Retraction. Aus rechtlichen Gründen kann der Zugriff nicht
gewährt werden.; Aachen, Techn. Hochsch., Diss., 2001},
abstract = {In this study the role of the GR1 gene in the race-specific
resistance of the Arabidopsis ecotype Ws to the P.
parasitica isolate WELA was investigated. In the susceptible
gr1 mutant GR1 is tagged by a T-DNA insert and it was
important to establish whether the mutant phenotype was
indeed due to the T-DNA insertion in GR1. Therefore, gr1
plants were retransformed using different genomic
constructs. Independent transformant lines showed local
resistance responses after inoculation with WELA and these
were not apparent in control plants. However, a completely
resistant transformant was not observed. An 8 kb genomic
fragment containing the GR1 gene was sequenced. The
predicted GR1 protein has a $57\%$ similarity to the ILR1
protein (Bartel und Fink, 1995), but not the same enzyme
activity. GR1 transcripts accumulated in leaves after
inoculation with WELA, wounding or treatment with
methyljasmonate. However, transcripts did not accumulate
after exposure to extreme temperatures, infection with P.
syringae pv. maculicola, or application of salicylic acid.
It was shown that the GR1 protein interacts with 14-3-3
proteins in a yeast-two-hybrid-assay, suggesting a potential
mechanism for GR1-mediated signal transmission. The analysis
of the GR1 gene and its product will lead to the
identification of further components of signal pathways
important in disease resistance and improve our
understanding of race-specific signal transduction in plant
defence.},
cin = {100000},
ddc = {570},
cid = {$I:(DE-82)100000_20140620$},
typ = {PUB:(DE-HGF)11},
urn = {urn:nbn:de:hbz:82-opus-1640},
doi = {10.18154/RWTH-CONV-118618},
url = {https://publications.rwth-aachen.de/record/56523},
}
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