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%0 Thesis
%A Jansen, Philipp
%T Endoglin (CD105) expression differentiates between aseptic loosening and periprosthetic joint infection after total joint arthroplasty
%I RWTH Aachen
%V Dissertation
%C Aachen
%M RWTH-2016-00974
%P 1 Online-Ressource (8 Seiten, 3 ungezählte Seiten) : Illustrationen, Diagramme
%D 2016
%Z Die Dissertation wurde zuvor 2014 als Aufsatz in der Zeitschrift "SpringerPlus" (3:561) veröffentlicht. - Auch veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2016
%Z Dissertation, RWTH Aachen, 2016
%X The differentiation between aseptic loosening and periprosthetic joint infection (PJI) after total joint arthroplastyis essential for successful therapy. A better understanding of pathogenesis of aseptic loosening and PJI may helpto prevent or treat these complications. Previous investigations revealed an increased vascularization in theperiprosthetic membrane in cases of PJI via PET signals. Based on these findings our hypothesis was that PJI isassociated with an increased neovascularization in the periprosthetic membrane.Tissue samples from periprosthetic membranes of the bone-implant interface were investigated histologically forinflammation, wear particles, vascularization and fibrosis. To identify vascular structures antibodies against CD 31,CD 34, factor VIII and CD 105 (endoglin) were applied for immunohistochemical investigations. According to aconsensus classification of Morawietz the tissue samples were divided into four types: type I (wear particle inducedtype, n = 11), type II (infectious type, n = 7), type III (combined type, n = 7) and type IV (indeterminate type, n = 7).Patients with PJI (type II) showed a pronounced infiltration of neutrophil granulocytes in the periprostheticmembrane and an enhanced neovascularization indicated by positive immunoreaction with antibodies against CD105 (endoglin). Tissue samples classified as type I, type III and type IV showed significantly less immune reaction forCD 105.In cases of aseptic loosening and PJI vascularization is found in different expression in periprosthetic membranes.However, in aseptic loosening, there is nearly no neovascularization with CD 105-positive immune reaction.Therefore, endoglin (CD 105) expression allows for differentiation between aseptic loosening and PJI.
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%U <Go to ISI:>//WOS:000359102500003
%$ pmid:26034672
%R 10.1186/2193-1801-3-561
%U https://publications.rwth-aachen.de/record/567789