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TY - THES AU - Terenzi, Carla TI - Novel strategies for the inhibition of biofilm formation on polymer surfaces CY - Aachen PB - Publikationsserver der RWTH Aachen University M1 - RWTH-CONV-123193 SP - XII, 110 S. : Ill., graph. Darst. PY - 2006 N1 - Aachen, Techn. Hochsch., Diss., 2006 AB - The aim of this work was the development of new strategies to prevent bacterial adhesion and biofilm formation on biomaterial surfaces. Two different anti-quorum sensing (QS) molecules, i.e. compounds that inhibit the communication system of bacteria, were employed: 3-butyl-5-(bromomethylene)-2(5H)-furanone and the RNA III inhibiting peptide (RIP). These molecules have a big advantage compared to the currently used antimicrobial agents, since they do not kill bacteria, they are unlikely to induce bacterial resistance. 3 Butyl 5 (bromomethylene) 2(5H) furanone is one of the secondary metabolites produced by the Australian macroalga Delisea pulchra to protect its surface from colonization and fouling by marine organisms. In order to mimic the defense mechanism evolved by the macroalga, the anti-QS molecule was synthesized and incorporated into films of the commercially available poly(D,L lactide) (PDLLA) (Resomer® 208), a polymer with long history in biomedical applications. Subsequently the release behavior of the halogenated furanone-loaded PDLLA films was investigated in an in vitro experiment. The RIP peptide (YSPWTNF) was used as an anti-biofilm coating for the non degradable polymer polyvinylidene fluoride (PVDF). The RIP peptide’s amide form was synthesized by solid phase peptide synthesis (SPPS). After isolation, purification and complete characterization, the anti QS-molecule was covalently coupled to the PVDF surface. As PVDF does not posses functional groups, which allow a surface modification, a plasma induced graft polymerization method was applied to activate and functionalize the polymer surface. Acrylic acid (AAc) was polymerized onto the surface of Argon-plasma activated PVDF. The bioligand RIP NH2 was then immobilized to the prepared carboxy functionalized PVDF-g-PAAc surface. Each step of the immobilization sequence was characterized by means of different surface sensitive techniques. Finally, the antibacterial properties of RIP NH2 coated PVDF surfaces were determined in vitro by means of a pico Green assay using Staphylococcus aureus (ATCC 29213). Another part of this work dealt with the encapsulation of Kathon® 910 SB, a formulation with a broad spectrum of activity against bacteria and fungi, into poly(dimethyl siloxane) (PDMS) microspheres. The antibacterial and antifungal properties of the prepared microspheres were investigated by means of dilution tests and agar diffusion hole tests. LB - PUB:(DE-HGF)11 UR - https://publications.rwth-aachen.de/record/61536 ER -