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@PHDTHESIS{Terenzi:61536,
      author       = {Terenzi, Carla},
      othercontributors = {Höcker, Hartwig},
      title        = {{N}ovel strategies for the inhibition of biofilm formation
                      on polymer surfaces},
      address      = {Aachen},
      publisher    = {Publikationsserver der RWTH Aachen University},
      reportid     = {RWTH-CONV-123193},
      pages        = {XII, 110 S. : Ill., graph. Darst.},
      year         = {2006},
      note         = {Aachen, Techn. Hochsch., Diss., 2006},
      abstract     = {The aim of this work was the development of new strategies
                      to prevent bacterial adhesion and biofilm formation on
                      biomaterial surfaces. Two different anti-quorum sensing (QS)
                      molecules, i.e. compounds that inhibit the communication
                      system of bacteria, were employed:
                      3-butyl-5-(bromomethylene)-2(5H)-furanone and the RNA III
                      inhibiting peptide (RIP). These molecules have a big
                      advantage compared to the currently used antimicrobial
                      agents, since they do not kill bacteria, they are unlikely
                      to induce bacterial resistance. 3 Butyl 5 (bromomethylene)
                      2(5H) furanone is one of the secondary metabolites produced
                      by the Australian macroalga Delisea pulchra to protect its
                      surface from colonization and fouling by marine organisms.
                      In order to mimic the defense mechanism evolved by the
                      macroalga, the anti-QS molecule was synthesized and
                      incorporated into films of the commercially available
                      poly(D,L lactide) (PDLLA) (Resomer® 208), a polymer with
                      long history in biomedical applications. Subsequently the
                      release behavior of the halogenated furanone-loaded PDLLA
                      films was investigated in an in vitro experiment. The RIP
                      peptide (YSPWTNF) was used as an anti-biofilm coating for
                      the non degradable polymer polyvinylidene fluoride (PVDF).
                      The RIP peptide’s amide form was synthesized by solid
                      phase peptide synthesis (SPPS). After isolation,
                      purification and complete characterization, the anti
                      QS-molecule was covalently coupled to the PVDF surface. As
                      PVDF does not posses functional groups, which allow a
                      surface modification, a plasma induced graft polymerization
                      method was applied to activate and functionalize the polymer
                      surface. Acrylic acid (AAc) was polymerized onto the surface
                      of Argon-plasma activated PVDF. The bioligand RIP NH2 was
                      then immobilized to the prepared carboxy functionalized
                      PVDF-g-PAAc surface. Each step of the immobilization
                      sequence was characterized by means of different surface
                      sensitive techniques. Finally, the antibacterial properties
                      of RIP NH2 coated PVDF surfaces were determined in vitro by
                      means of a pico Green assay using Staphylococcus aureus
                      (ATCC 29213). Another part of this work dealt with the
                      encapsulation of Kathon® 910 SB, a formulation with a broad
                      spectrum of activity against bacteria and fungi, into
                      poly(dimethyl siloxane) (PDMS) microspheres. The
                      antibacterial and antifungal properties of the prepared
                      microspheres were investigated by means of dilution tests
                      and agar diffusion hole tests.},
      cin          = {154720 / 150000},
      ddc          = {570},
      cid          = {$I:(DE-82)154720_20140620$ / $I:(DE-82)150000_20140620$},
      typ          = {PUB:(DE-HGF)11},
      urn          = {urn:nbn:de:hbz:82-opus-17284},
      url          = {https://publications.rwth-aachen.de/record/61536},
}