h1

TY  - THES
AU  - Jeude, Markus
TI  - Entwicklung und Anwendung einer Fed-batch-Betriebsweise mit Nährstofffreisetzungssystemen zur kontrollierten Kultivierung und zum Screening von Mikroorganismen in Schüttelreaktoren
CY  - Aachen
PB  - Publikationsserver der RWTH Aachen University
M1  - RWTH-CONV-124003
SP  - XVII, 262 S. : Ill., graph. Darst.
PY  - 2007
N1  - Aachen, Techn. Hochsch., Diss., 2007
AB  - Most industrial production processes are performed in fed-batch operational mode. In contrast, the screenings for microbial production strains are run in batch mode which results in completely different physiological conditions than relevant for production. This may lead to wrong strain selections. Silicone elastomer discs containing glucose crystals were developed to realize fed-batch fermentation based on diffusion in shaken bioreactors. No other device for feeding was required. This “slow-release fed-batch technique” was tested on the metabolism of H. polymorpha, E. coli and G. oxydans in shake flasks. The OTR and RQ were monitored online with a RAMOS device. Biomass formation, synthesis of proteins like GFP or eYFP-IL-6, pH drift and metabolic dynamics of glucose, ethanol, acetic acid and other organic acids were measured offline. By application of the slow-release fed-batch technique in comparison to regular batch mode, overflow-metabolism of H. polymorpha and E. coli could be reduced, which led to an increase in biomass yield of up to 85
KW  - Fed-batch-Verfahren (SWD)
KW  - Schüttelkolben (SWD)
KW  - Hansenula polymorpha (SWD)
KW  - Escherichia coli (SWD)
KW  - Gluconobacter oxydans (SWD)
KW  - Diffusion (SWD)
KW  - Glucose (SWD)
KW  - Grün fluoreszierendes Protein (SWD)
KW  - Freisetzung (SWD)
LB  - PUB:(DE-HGF)11
UR  - https://publications.rwth-aachen.de/record/62434
ER  -