h1

%0 Thesis
%A Unthan, Simon
%T Robot-assisted phenotyping of genome-reduced Corynebacterium glutamicum strain libraries to draft a chassis organism
%V 132
%I RWTH Aachen University
%V Dissertation
%C Jülich
%M RWTH-2016-07483
%B Schriften des Forschungszentrums Jülich. Reihe Schlüsseltechnologien / Key Technologies
%P 1 Online-Ressource (122 Seiten) : Illustrationen, Diagramme
%D 2016
%Z Druckausgabe: 2016. - Onlineausgabe: 2016. - Auch veröffentlicht auf dem Publikationsserver der RWTH Aachen University
%Z Dissertation, RWTH Aachen University, 2015
%X In this work, concepts were developed and applied to guide the construction of a Corynebacterium glutamicum chassis organism for synthetic biology approaches. The aim was to delete irrelevant genes from the wild type strain in order to obtain a chassis growing on defined CGXII medium with D-glucose with unaltered biological fitness, which was defined by the maximum specific growth rate and biomass yield.Initially, workflows were developed on a robotic Mini Pilot Plant (MPP), for example, to harvest cell-free cultivation supernatants from BioLector cultivations in response to individually defined triggers. Subsequently, assays for amino acids and D-glucose were established in 384-well plate scale in order to quantify these metabolites in cell-free culture supernatants in fully automated workflows [1].During initial reference experiments, protocatechuic acid was identified as a hidden co-substrate in the well-known defined CGXII medium. The additional TCA feed via acetyl-CoA and succinyl-CoA, which are derived from protocatechuic acid, elevates the growth rate by about 50 
%F PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
%9 Dissertation / PhD ThesisBook
%U https://publications.rwth-aachen.de/record/670366