h1

TY  - THES
AU  - Giesbertz, Anna
TI  - Triple helix-targeted DNA methylation with DNA methyltransferase-oligodeoxynucleotide conjugates
PB  - RWTH Aachen University
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2019-00264
SP  - 1 Online-Ressource (VII, 104 Seiten) : Illustrationen, Diagramme
PY  - 2018
N1  - Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2019
N1  - Dissertation, RWTH Aachen University, 2018
AB  - In this work, three bacterial DNA methyltransferases (MTases), that methylate cytosine within the CpG recognition sequence, were coupled to a triple helix-forming oligodeoxynucleotide (TFO) to create constructs for targeted DNA methylation. The constructs were designed to target and methylate a single CpG site located in the promoter region of the epithelial cell adhesion molecule (EpCAM), of which the overexpression has been found in many carcinomas and is correlated to the methylation status within the promoter region (van der Gun et al., 2008). The coupling strategy was based on the SNAP-tag technology, with which the benzylic carbon of a para-substituted benzylguanine (BG) is connected to a variant of O6-alkylguanine DNA alkyltransferase (hAGT) that is fused with a DNA MTase. The BG modification of the TFO occurred in two steps and BG-PEG35-TFO was obtained with an overall yield of 65
LB  - PUB:(DE-HGF)11
DO  - DOI:10.18154/RWTH-2019-00264
UR  - https://publications.rwth-aachen.de/record/752832
ER  -