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%0 Thesis
%A Wings, Tina Katrin
%T Charakterisierung landwirtschaftlicher Abwässer im Hinblick auf mikrobiologische Risikofaktoren zur Beurteilung des Einflusses von Biogasanlagen, insbesondere ihrer Barrierefunktion, auf Förderung und Verbreitung von Resistenzgenen sowie resistenter und pathogener Bakterien
%I RWTH Aachen University
%V Dissertation
%C Aachen
%M RWTH-2019-09081
%P 1 Online-Ressource (vi, 156 Seiten) : Illustrationen, Diagramme
%D 2017
%Z Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2019
%Z Dissertation, RWTH Aachen University, 2017
%X Continuous expansion and intensification of livestock farming facilitates large quantities of agricultural waste which in turn are released into the environment. The application of untreated manure on agricultural land, however, in addition to a valuable nutrient supply, constitutes a direct pathway for veterinary pharmacological trace substances, heavy metal residues, pathogenic and resistant microorganisms and into soil, groundwater and surface water. This study examines the distribution and persistence of antimicrobial resistance genes (ARG), resistant and potentially pathogenic microorganisms and heavy metals with antibiotic properties in agricultural effluents ascertaining the risk of manure spreading on farmland and the sanitizing or rather retaining potential of biogas plants. 15 biogas plants were examined in a screening of random samples in 2012 of which three plants were monitored over the annual cycle in 2013 with five sampling times. Continuous flow fermenters were investigated under defined addition of antibiotics (AB) in the first half of the experiment against a control fermenter over a total period of 60 days. Micro- and molecular biological approaches show a generally low prevalence of anaerobic pathogenic bacteria as well as a reduction of cultivatable bacteria through the process of biogas production, including clinically relevant organisms of Enterobacteriaceae and Enterococcaceae without promoting anaerobic spore forming pathogens. Isolates show a decreasing diversity and the reduction of gram negative bacteria in the output samples and a shift of the bacterial community in favor of the phylum Firmicutes. Although antibiotic resistance genes (ARG) can be quantified both ubiquitous in manure and digestate, a decline of resistant bacteria and ARG can be detected after fermentation. However, the quantitative analysis also illustrates biogas plant and ARG specific levels and reduction efficiencies between 80 
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%R 10.18154/RWTH-2019-09081
%U https://publications.rwth-aachen.de/record/767823