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TY  - THES
AU  - Loevenich, Johanna
TI  - Optimization of itaconic acid production by U. maydis through metabolic engineering </td><td width="150">
TI  - amp; adaptive laboratory evolution; 1. Auflage
VL  - 14
PB  - RWTH Aachen University
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2020-01372
T2  - Applied microbiology
SP  - 1 Online-Ressource (XIV, 123 Seiten) : Illustrationen, Diagramme
PY  - 2019
N1  - Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2020
N1  - Dissertation, RWTH Aachen University, 2019
AB  - The incessant growth of the world population leads to an already gigantic and still increasing demand for food, energy, fuels, and chemicals. With the finiteness of fossil resources as main feedstocks, a change from petroleum-derived to sustainable, economically bio-based production processes is indispensable to accomplish the global needs. One of these processes is the production of itaconic acid ranked as one of the top 12 value added chemicals from biomass by the DoE. Nowadays, industrial biotechnological production is performed by using the filamentous fungus Aspergillus terreus. To circumvent the challenges going along with such a filamentous production host, alternatives are searched. In this context, the Ustilaginaceae family including Ustilago maydis attracted special attention. To establish an industrial itaconate production host competitive to A. terreus and to significantly improve the itaconate production performance of U. maydis, two strategies were chased in this thesis: metabolic engineering and adaptive laboratory evolution. By the reduction of the diverse by-product spectrum of U. maydis MB215 by the deletion of 2-hydroxy paraconate, mannosyl-erythritol lipid, ustilagic acid and triacylglycerol biosynthesis in combination with the upregulation of ria1, the itaconate biosynthesis gene cluster regulator, the flow of substrate could be extensively pushed towards itaconate biosynthesis. This lead to an itaconate titer increased by 10.2-fold compared to the wildtype. Due to the upregulation of the cis-aconitate/malate antiporter mtt1 as consequence of ria1↑, the production of malate, another by-product, could simultaneously be decreased by 84 
LB  - PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
DO  - DOI:10.18154/RWTH-2020-01372
UR  - https://publications.rwth-aachen.de/record/781564
ER  -