h1

TY  - THES
AU  - Lenzen, Christoph
TI  - Metabolic engineering of Pseudomonas taiwanensis VLB120 for sustainable production of 4-Hydroxybenzoate; 1. Auflage
VL  - 17
PB  - RWTH Aachen University
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2020-02978
T2  - Applied microbiology
SP  - 1 Online-Ressource (XVIII, 153 Seiten) : Illustrationen, Diagramme
PY  - 2020
N1  - Auch veröffentlicht auf dem Publikationsserver der RWTH Aachen University
N1  - Dissertation, RWTH Aachen University, 2019
AB  - The aromatic compound 4-hydroxybenzoate and its derivatives, the parabens, find applications in everyday life. Current production routes for aromatics such as 4-hydroxybenzoate are mainly based on chemical catalysis and depend on the intensive use of energy and fossil resources. Due to the decreasing availability of the latter, the rising demand for aromatics and the non-ecofriendly way of formation, there is an urgent need for finding more efficient and sustainable syntheses. The present work focused on the development of a Pseudomonas-based whole-cell biocatalyst for the bioconversion of 4-hydroxybenzoate from renewable substrates such as glucose or glycerol. Besides the remarkable and versatile metabolism of this genus and its native high tolerance towards toxic compounds such as solvents, the species Pseudomonas taiwanensis VLB120 was chosen as a production host, since it accepts five carbon sugars such as xylose as sole carbon source and it is not regarded as a pathogenic organism. Former attempts to biotechnologically produce 4-hydroxybenzoate using different Pseudomonads and other species were indeed successful, but several studies resulted in only minor yields or required the supplementation of additional metabolites due to auxotrophies. Therefore, the objective was to enable high-yield 4-hydroxybenzoate biosynthesis solely from one specific carbon source. In order to exploit the full potential of metabolic engineering, rational as well as non-rational techniques were applied for host development. By introducing a heterologous production pathway based on tyrosine, eliminating and downregulating competing pathways and overexpressing key genes, strain P. taiwanensis VLB120 CL4.3 produced 4-hydroxybenzoate with a C-mol yield of 19.0
LB  - PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
DO  - DOI:10.18154/RWTH-2020-02978
UR  - https://publications.rwth-aachen.de/record/785128
ER  -