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@MISC{Weingartner:785777,
      author       = {Weingartner, Alexandra Maria and Ruff, Anna Joelle and
                      Schwaneberg, Ulrich and Davari, Mehdi D. and Sauer, Daniel
                      F. and Dhoke, Gaurao V.},
      title        = {{D}ataset to "{A} hydroquinone-specific screening system
                      for directed {P}450 evolution"},
      reportid     = {RWTH-2020-03484},
      year         = {2020},
      abstract     = {In direct evolution, an effective screening strategy is
                      crucial for the identification of improved enzymes with
                      desired characteristics in large mutant libraries. In the
                      manuscript “A hydroquinone specific screening system for
                      directed enzyme evolution” (A. M. Weingartner, D. F.
                      Sauer, G. V. Dhoke, M. D. Davari, A. J. Ruff, and U.
                      Schwaneberg, Appl Microbiol Biotechnol. 2018; 102(22):
                      9657–9667. doi: 10.1007/s00253-018-9328-3), we report a
                      first screening system designed for screening of P450
                      variants capable to produce hydroquinones. The hydroquinone
                      quantification assay is based on the interaction of
                      4-Nitrophenylacetonitrile (NpCN) with hydroquinones under
                      alkaline conditions. The NpCN assay can be used for the
                      quantification of dihydroxylated aromatic compounds such as
                      hydroquinones, catechols and benzoquinones. We chose the
                      hydroxylation of pseudocumene by P450 BM3 as a target
                      reaction and screened for improved trimethylhydroquinone
                      (TMHQ) formation. Here the dataset to the above mentioned
                      manuscript is reported. Source Data of the NMR spectra (Fig1
                      and FigS1) are given as well as source data in Excel format
                      to display the spectra of the hydroquinones (FigS3), the
                      linear detection range of the assay of different
                      hydroquinones (FigS6) or of TMHQ (Fig2) and screening data
                      of the SSM library on position 330 with the NpCN assay
                      (FigS7). In the description of dataset a summary of the
                      collected source data is given.},
      cin          = {162610 / 160000},
      cid          = {$I:(DE-82)162610_20140620$ / $I:(DE-82)160000_20140620$},
      pnm          = {ROBOX - Expanding the industrial use of Robust Oxidative
                      Biocatalysts for the conversion and production of alcohols
                      (ROBOX) (635734)},
      pid          = {G:(EU-Grant)635734},
      typ          = {PUB:(DE-HGF)32},
      doi          = {10.18154/RWTH-2020-03484},
      url          = {https://publications.rwth-aachen.de/record/785777},
}