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@PHDTHESIS{Kerth:828162,
      author       = {Kerth, Janna-Lina},
      othercontributors = {Orlikowsky, Thorsten and Stickeler, Elmar},
      title        = {{P}eriphagozytäre {R}eaktionen bei neonatalen und adulten
                      {M}onozyten nach {D}oppelinfektion mit {E}. coli},
      school       = {Rheinisch-Westfälische Technische Hochschule Aachen},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2021-08976},
      pages        = {1 Online-Ressource : Illustrationen},
      year         = {2021},
      note         = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
                      University; Dissertation, Rheinisch-Westfälische Technische
                      Hochschule Aachen, 2021},
      abstract     = {Neonatal sepsis is the most important cause for neonatal
                      deaths; E. coli is the second most common pathogen. In the
                      neonatal, immature immune system, monocytes are, amongst
                      others, responsible for early elimination of pathogens. This
                      especially holds true for bacteremia and sepsis. After
                      phagocytosis, monocytes secrete various pro- and
                      anti-inflammatory cytokines and soluble factors which also
                      induce the so called bystander kill. In an in vitro model,
                      we simulated a two-time infection with E. coli. We
                      postulated differences between neonatal and adult monocytes
                      regarding phagocytic capacity, apoptosis rates and
                      bystanderkill, the ability for double phagocytosis, cytokine
                      secretion as well as killing of bacteria after phagocytosis.
                      Monocytes were harvested from cord blood and peripheral
                      blood of healthy adults and identified via FACS. Monocytes
                      were infected with fluorescent E. coli on two subsequent
                      days; afterwards, apoptosis rates were determined using
                      Nicoletti or Annexin-V staining, respectively, and
                      phagocytosis rates were determined using FACS. The
                      effectiveness of killing was measured through killing
                      assays, the secretion of IL-10 and TNF-alpha via ELISA. Both
                      CBMO and PBMO are capable of double phagocytosis. The
                      apoptosis rate of PBMO is higher than that of CBMO. CBMO
                      have a significantly higher phagocytic capacity than PBMO.
                      Apoptosis of CBMO was reduced after contact with the
                      pathogen. Double infection led to a more efficient killing
                      of bacteria in both groups. Measured by the
                      IL-10-TNF-alpha-ratio, infection led to an increased
                      secretion of TNF-alpha and a decreased production of IL-10
                      in CBMO. While our investigation is not without limitations,
                      our results point to a possible contribution to sustained
                      inflammation through an increased pro-inflammatory secretion
                      of cytokines in a prolonged infection in the cord blood.},
      cin          = {537500-2},
      ddc          = {610},
      cid          = {$I:(DE-82)537500-2_20140620$},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2021-08976},
      url          = {https://publications.rwth-aachen.de/record/828162},
}