h1

h2

h3

h4

h5
h6
%0 Thesis
%A Shokoohimehr, Pegah
%T Nanostraw- Nanocavity MEAs as a new tool for long-term and high sensitive recording of neuronal signals
%V 76
%I RWTH Aachen University
%V Dissertation
%C Jülich
%M RWTH-2021-11307
%@ 978-3-95806-593-2
%B Schriften des Forschungszentrums Jülich. Reihe Information
%P 1 Online-Ressource : Illustrationen
%D 2021
%Z Druckausgabe: 2021. - Onlineausgabe: 2021. - Auch veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2022
%Z Dissertation, RWTH Aachen University, 2021
%X Electrical measurement of neuronal signals has enabled fundamental discoveries in neuroscience. Patch clamp method as a key standard of electrophysiological device has been shown an access to the interior single cell using an electrode. Via this method recording of the signals from the entire spectrum of the membrane potentials, from action potential down to sub-threshold signals such as post synaptic potentials, is feasible. Due to the invasive nature of this method, long term recording of the cell is challenging. Extracellular electrodes, such as microelectrode arrays, in contrast enable long term recordings of neuronal networks. However, these electrodes can only measure a fraction of the action potentials, which is due to the lack of proper cell-electrode coupling and high noise of the electrodes. Research in the last decade has been focused on overcoming these limitations. Development of the vertical 3D nanoelectrodes has allowed to access the cell’s interior, however in most cases after the application of external forces such as opto/electro-poration, and therefore these transient methods are not suitable for long term recordings.In this thesis, I developed nanostructure microelectrodes by associating two approaches of nanostraws and nanocavities. Using nanostraws facilitate penetration to the cell membrane, and the introduction of nanocavities provide high seal-resistance. The spontaneous electrophysiological recording using our nanoelectrodes demonstrate both extracellular and intracellular (20
%F PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
%9 Dissertation / PhD ThesisBook
%R 10.18154/RWTH-2021-11307
%U https://publications.rwth-aachen.de/record/836436