% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @PHDTHESIS{Shokoohimehr:836436, author = {Shokoohimehr, Pegah}, othercontributors = {Offenhäusser, Andreas and Fitter, Joerg}, title = {{N}anostraw- {N}anocavity {MEA}s as a new tool for long-term and high sensitive recording of neuronal signals}, volume = {76}, school = {RWTH Aachen University}, type = {Dissertation}, address = {Jülich}, publisher = {Forschungszentrum Jülich GmbH, Zentralbibliothek, Verlag}, reportid = {RWTH-2021-11307}, isbn = {978-3-95806-593-2}, series = {Schriften des Forschungszentrums Jülich. Reihe Information}, pages = {1 Online-Ressource : Illustrationen}, year = {2021}, note = {Druckausgabe: 2021. - Onlineausgabe: 2021. - Auch veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2022; Dissertation, RWTH Aachen University, 2021}, abstract = {Electrical measurement of neuronal signals has enabled fundamental discoveries in neuroscience. Patch clamp method as a key standard of electrophysiological device has been shown an access to the interior single cell using an electrode. Via this method recording of the signals from the entire spectrum of the membrane potentials, from action potential down to sub-threshold signals such as post synaptic potentials, is feasible. Due to the invasive nature of this method, long term recording of the cell is challenging. Extracellular electrodes, such as microelectrode arrays, in contrast enable long term recordings of neuronal networks. However, these electrodes can only measure a fraction of the action potentials, which is due to the lack of proper cell-electrode coupling and high noise of the electrodes. Research in the last decade has been focused on overcoming these limitations. Development of the vertical 3D nanoelectrodes has allowed to access the cell’s interior, however in most cases after the application of external forces such as opto/electro-poration, and therefore these transient methods are not suitable for long term recordings.In this thesis, I developed nanostructure microelectrodes by associating two approaches of nanostraws and nanocavities. Using nanostraws facilitate penetration to the cell membrane, and the introduction of nanocavities provide high seal-resistance. The spontaneous electrophysiological recording using our nanoelectrodes demonstrate both extracellular and intracellular $(20\%$ of cases) action potentials of cortical rat neurons over long period of time. This approach enables the continuous high signal to noise ratio recordings with high sensitivity and the ability to record post synaptic potentials. To further improve the spatial resolution of neuronal network recordings, our nanoelectrodes can be integrated to CMOS-devices, which is of great interest for the neurophysiological studies.}, cin = {134210 / 130000}, ddc = {530}, cid = {$I:(DE-82)134210_20140620$ / $I:(DE-82)130000_20140620$}, typ = {PUB:(DE-HGF)11 / PUB:(DE-HGF)3}, doi = {10.18154/RWTH-2021-11307}, url = {https://publications.rwth-aachen.de/record/836436}, }