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TY - THES AU - Sivagnanam, Nirosha TI - Subzelluläre Lokalisation von BASIC (bile acid sensitive ion channel) PB - Rheinisch-Westfälische Technische Hochschule Aachen VL - Dissertation CY - Aachen M1 - RWTH-2022-00600 SP - 1 Online-Ressource : Illustrationen, Diagramme PY - 2021 N1 - Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2022 N1 - Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2021 AB - The bile acid-sensitive ion channel (BASIC) is a member of the degenerin/epithelial Na+ channel (Deg/ENaC) gene family. It is expressed mainly in brain, liver and intestine. In the liver the expression is limited on the cholangiocytes and in the brain on unipolar brush cells. As it‘s name bile acid-sensitive ion channel implies, BASIC is activated by bile acids. The physiological function of BASIC is yet unknown.In this study the subcellular localisation of BASIC was examined. After transient transfection of BASIC in heterologous cell lines HEK293 and CHO, a reticular distribution pattern has been observed. To identify this reticular distribution pattern, cells were stained with fluorescent organelle markers or antibodies and were examined with confocal microscopy. In the process, a colocalisation of BASIC and anti-PDI, an antibody against endoplasmtic reticulum, has been found. In order, the protein sequence was analysed to identify motives that could be the reason for the distribution pattern of BASIC in the cell. BASIC consists of two transmembrane domains, an extracellular loop and short intracellular amino- and carboxy-termini. Some truncations on the amino- and carboxy-terminus were made, which did not cause any differences in the reticular distribution pattern of BASIC. Therefore no motive could be determined as important for the targeting of BASIC. A staining of the plasma membrane was also not detected. In summary, this study could identify a localisation of BASIC in endoplasmatic reticulum. So it can be suggested that BASIC plays a role in this intracellular compartment. LB - PUB:(DE-HGF)11 DO - DOI:10.18154/RWTH-2022-00600 UR - https://publications.rwth-aachen.de/record/838450 ER -