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@PHDTHESIS{Svetlova:841188,
      author       = {Svetlova, Anastasia},
      othercontributors = {Offenhäusser, Andreas and Pich, Andrij},
      title        = {{B}iomimetic coatings on technical substrates for cell
                      culture},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2022-01542},
      pages        = {1 Online-Ressource : Illustrationen, Diagramme},
      year         = {2022},
      note         = {Englische und deutsche Zusammenfassung. - Veröffentlicht
                      auf dem Publikationsserver der RWTH Aachen University;
                      Dissertation, RWTH Aachen University, 2022},
      abstract     = {Ability to study living cells by a non-invasive methods,
                      such as cell culture, was a foundation for modern biological
                      and biotechnological advances. As it was discovered that a
                      cell function cannot be separated from its surroundings, a
                      quest to recreate living tissues in controlled laboratory
                      conditions began. The researchers are constantly trying to
                      impove methodology to study cells in environments that
                      resemble natural tissue environments the most. The creation
                      of complex environments requires precise controll of
                      properties of the underlying substrate. The project
                      consisted of the development of the coating that mimics a
                      natural cell membrane and is a suitable platform for cell
                      culture, and the adaptation of the electrical measurement
                      system. Solid-supported lipid bilayers (SLBs) were used as
                      the basis for the biomimetic coatings, and graphene
                      field-effect transistors (GFETs) as the measurement system.
                      The major challenge of the first part of the project was to
                      overcome hydrophobicity of graphene surface, as lipid
                      bilayers are not formed on the pristine graphene. An
                      electrochemical oxidation was tested as a route to overcome
                      that. However, it was revealed that chemical vapor deposited
                      (CVD) graphene is incompatible with this method, as the
                      process, instead of the uniform functionalization,
                      destructed the layer integrity. Moving along with the theme
                      of a link between electrochemical properties and the surface
                      quality, the detailed research on the origins of the leakage
                      currents of graphene electrolyte-gated transistors was
                      performed. It was determined that value can be used to
                      evaluate and monitor surface properties of graphene in situ.
                      Results of these findings were summarized in publications
                      [1, 2].To produce biomimetic coatings, extracts from cell
                      membranes of HEK293 cells were made by the chemical
                      vesiculation method. Similarity of the structural backbone
                      of cell vesicles and SLBs made from artificial lipid mixes
                      allows them to fuse into composite layers that are enriched
                      by cell membrane molecules, such as glycolipids and
                      proteins, that are important for cell-cell interactions. The
                      chemical vesiculation process was studied with the flow
                      cytometry and the fluorescent imaging methods, and fusion of
                      layers – by the quartz crystal microbalance in a
                      microfluidic system. Two cell culture models were used to
                      characterize their interactions with the biomimetic layers:
                      HL-1, the cardiomyocyte-like cell line, and primary cortical
                      neurons from rat embryo brains. These cells have distinctly
                      different properties and demands to their environment, so
                      different characterization pathways were selected for each.
                      For HL-1 cells, which are, essentially, muscle cells, their
                      cytoskeleton was monitored. The ability to form a developed
                      cytoskeleton that supports the cell shape and contractions
                      depends on the strength of their interaction with the
                      substrate and its softness. Biomimetic coatings modified
                      with different amounts of cell extracts allowed to tune the
                      optimal conditions for cell adhesion. These findings were
                      published in [3].For neuronal cells in vitro the development
                      of the cell polarity is their main property. The neurite
                      elongation dynamics on different coatings were analyzed and
                      it was discovered that interactions of neurons with highly
                      fluid SLB-based coatings have unique features during the
                      earliest hours of the development.},
      cin          = {134210 / 130000 / 163820 / 160000},
      ddc          = {530},
      cid          = {$I:(DE-82)134210_20140620$ / $I:(DE-82)130000_20140620$ /
                      $I:(DE-82)163820_20140620$ / $I:(DE-82)160000_20140620$},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2022-01542},
      url          = {https://publications.rwth-aachen.de/record/841188},
}