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TY  - THES
AU  - Schöps, Malte
TI  - Design, verification and validation of a novel large-volume production of ghost cells
VL  - 68
PB  - RWTH Aachen University
VL  - Dissertation
CY  - Düren
M1  - RWTH-2022-04263
SN  - 978-3-8440-8543-3
T2  - Aachener Beiträge zur Medizintechnik
SP  - XI, 122 Seiten : Illustrationen, Diagramme
PY  - 2022
N1  - Abweichender Titel auf dem Buchrücken
N1  - Dissertation, RWTH Aachen University, 2021
AB  - (1) Mechanical circulatory support is mainly based on moving actuators in the organism blood, such as the blades of centrifugal blood pumps. Using these devices in the treatment of patients exposes blood to unusual stress, causing hemolysis. Hemolysis is still one of the major challenges in the development of mechanical circulatory support devices, apart from thrombocyte activation. Close to the surface between rotor and blood, high shear stress acts on red blood cells. As soon as shear stress exceeds a threshold value, it leads to hemolysis, destroying red blood cells by rupturing their membranes. (2) The fluorescent hemolysis detection method (FHDM) developed by Jansen et al. investigates hemolysis in more detail. With this method, a spatial resolution of hemolysis hotspots is realized based on ghost cells. Ghost cells are red blood cells with reduced intracellular hemoglobin. The FHDM is limited by the small amounts of ghost cells produced. Larger volumes would allow to perform this method even according to international standards and on real size models of mechanical circulatory support systems. The aim of this study was to develop a process engineering system using semi-automatic mechatronic technology to increase ghost cell production volume. (3) Until now, production volume was limited to 10.3 mL of ghost cells with a hematocrit of 30 
LB  - PUB:(DE-HGF)11 ; PUB:(DE-HGF)3
DO  - DOI:10.2370/9783844085433
UR  - https://publications.rwth-aachen.de/record/844852
ER  -