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@PHDTHESIS{Schps:844852,
author = {Schöps, Malte},
othercontributors = {Steinseifer, Ulrich and Jupke, Andreas},
title = {{D}esign, verification and validation of a novel
large-volume production of ghost cells},
volume = {68},
school = {RWTH Aachen University},
type = {Dissertation},
address = {Düren},
publisher = {Shaker Verlag},
reportid = {RWTH-2022-04263},
isbn = {978-3-8440-8543-3},
series = {Aachener Beiträge zur Medizintechnik},
pages = {XI, 122 Seiten : Illustrationen, Diagramme},
year = {2022},
note = {Abweichender Titel auf dem Buchrücken; Dissertation, RWTH
Aachen University, 2021},
abstract = {(1) Mechanical circulatory support is mainly based on
moving actuators in the organism blood, such as the blades
of centrifugal blood pumps. Using these devices in the
treatment of patients exposes blood to unusual stress,
causing hemolysis. Hemolysis is still one of the major
challenges in the development of mechanical circulatory
support devices, apart from thrombocyte activation. Close to
the surface between rotor and blood, high shear stress acts
on red blood cells. As soon as shear stress exceeds a
threshold value, it leads to hemolysis, destroying red blood
cells by rupturing their membranes. (2) The fluorescent
hemolysis detection method (FHDM) developed by Jansen et al.
investigates hemolysis in more detail. With this method, a
spatial resolution of hemolysis hotspots is realized based
on ghost cells. Ghost cells are red blood cells with reduced
intracellular hemoglobin. The FHDM is limited by the small
amounts of ghost cells produced. Larger volumes would allow
to perform this method even according to international
standards and on real size models of mechanical circulatory
support systems. The aim of this study was to develop a
process engineering system using semi-automatic mechatronic
technology to increase ghost cell production volume. (3)
Until now, production volume was limited to 10.3 mL of ghost
cells with a hematocrit of 30 $\%$ due to predominantly
manual process steps. By implementation of a novel
semi-automated large-volume batch production system (LVBPS)
in the existing method, productivity was increased 22-fold
while multiplying process efficiency by 34 times.
Time-consuming manual work such as pipetting was supported
by sensor-based process engineering. In addition to
increased efficiency, process monitoring was implemented in
the system to ensure consistent process parameters and
semi-automation of the production process. Moreover, the
properties of ghost cells as blood substitute such as
rheology and deformability were maintained or even enhanced
compared to manual production. With the help of the LVBPS,
the objective of producing large volumes of ghost cells was
successfully achieved.},
cin = {811001-1 / 416310},
ddc = {620},
cid = {$I:(DE-82)811001-1_20140620$ / $I:(DE-82)416310_20151215$},
pnm = {Fluorescent Hemolysis Detection (FHD): Validierung der
In-vitro Testmethode (321130633)},
pid = {G:(GEPRIS)321130633},
typ = {PUB:(DE-HGF)11 / PUB:(DE-HGF)3},
doi = {10.2370/9783844085433},
url = {https://publications.rwth-aachen.de/record/844852},
}
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