h1

TY  - THES
AU  - Weisner, Danika
TI  - Dibenzazepin assoziierte sekretorische Transformation und Bakterizidie enterozytärer Zellen
PB  - Rheinisch-Westfälische Technische Hochschule Aachen
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2022-08025
SP  - 1 Online-Ressource : Illustrationen, Diagramme
PY  - 2022
N1  - Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2023
N1  - Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2022
AB  - The regulation of the intestinal epithelia is controlled by several signal pathways (f.e. Notch and Wnt), additional regulators include Caspase8. A disbalance can affect the composition and performance of the epithelia and lead to different pathologies such as intestinal infections.This dissertation set out to analyse changes to the antibacterial functionality of secretory differentiation after inhibition of the Notch Pathway by treatment with DBZ. Antibacterial functionality was measured by performing an antibacterial assay. To analyse the antibacterial function after treatment with DBZ in murine intestine the Weiser method was successfully applied, separating the epithelia in three different compartments along the crypt-villus-axis (villi, apical crypts, basal crypts). The study showed an increase in antibacterial functionality in the villi and a difference between the compartments was found, though it gives no clear answer to the cellular origin of the increase.In CaCo2 DBZ treatment increases the antibacterial functionality in wt und CaCo2∆Casp8¬ dependant on the treatment time. Colo320 showed no change to bacterial growth after DBZ treatment or loss of Caspase8. The results show that DBZ treatment of colonic enterocytes can induce antibacterial functionality, dependant on the characteristics and behaviour of the cells, though not dependant on Caspase8. In summary, this study showed that DBZ treatment can induce bactericity in mice and cellular culture with characteristics of small intestinal epithelia. The secretory transdifferentiation in mice after DBZ treatment results in increased antimicrobial function. The study showed regional differences of the antibacterial functionality along the crypt-villus-axis, this finding needs to be confirmed in an extension to the performed experiments. Changes to DBZ effect after ∆Casp8 in mice were not shown in CaCo2. It appears that DBZ induced bactericity is dependent on the characteristic and potential of the epithelia.
LB  - PUB:(DE-HGF)11
DO  - DOI:10.18154/RWTH-2022-08025
UR  - https://publications.rwth-aachen.de/record/852369
ER  -