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@PHDTHESIS{Hengsbach:960882,
      author       = {Hengsbach, Rebecca},
      othercontributors = {Simon, Ulrich and Wöll, Dominik},
      title        = {{M}ultifunktionale {M}ikrogel-{DNA}-{H}ybridsysteme},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2023-06486},
      pages        = {1 Online-Ressource : Illustrationen, Diagramme},
      year         = {2023},
      note         = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
                      University; Dissertation, RWTH Aachen University, 2023},
      abstract     = {Within this work multifunctional microgel-DNA-hybrid
                      systems were created and investigated. The covalent binding
                      of DNA inside of microgels leads to a combination of their
                      properties which is of great interest regarding biomedical
                      application. The first part of this work showed a changed
                      volume phase transition of microgels by incorporation of the
                      DNA. This was analyzed by NMR measurements. Regarding
                      biomedical applications the phase transition was examined in
                      different concentrations of NaCl as well as in PBS. It was
                      shown that the phase transition is around body temperature
                      in PBS. In the second part of this work a regioselective DNA
                      functionalization of microgels in the inside was examined.
                      For this core-shell microgels were created. The core
                      microgels did contain disulfides, which were used for the
                      DNA functionalization. An adjustment of the thickness of the
                      shell was achieved by variation of the synthesis. The
                      localization of the disulfides was shown via dSTORM and the
                      localization of the DNA functionalization was examined via
                      DNA-PAINT. In both cases a localization of the dyes was
                      shown in the inner parts of the microgels but not restricted
                      to the core only. In the third part of this work substrates
                      for a targeted immobilization of DNA functionalized
                      microgels in their native structure were developed.
                      Commercially available and own ITO substrates were compared
                      and 2-(4-chlorosulfonylphenyle)ethyltrimethoxy-silane as
                      well as a comparable silane-derivate synthesized at the
                      surface were used. The production of ITO substrates and the
                      surface bound synthesis of the silane-derivate should have
                      been optimized and showed nice results. A localized labeling
                      with dyes inside of a pattern produced via chemical electron
                      beam lithography was partially successful.},
      cin          = {151310 / 150000},
      ddc          = {540},
      cid          = {$I:(DE-82)151310_20140620$ / $I:(DE-82)150000_20140620$},
      pnm          = {SFB 985 A06 - Oberflächengebundene Mikrogele und
                      Mikrogel-Überstrukturen – Synthese, Schaltverhalten und
                      Bildgebung (A06) (221468158) / DFG project 191948804 - SFB
                      985: Funktionelle Mikrogele und Mikrogelsysteme (191948804)},
      pid          = {G:(GEPRIS)221468158 / G:(GEPRIS)191948804},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2023-06486},
      url          = {https://publications.rwth-aachen.de/record/960882},
}