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@PHDTHESIS{Vedaraman:961720,
      author       = {Vedaraman, Sitara},
      othercontributors = {De Laporte, Laura and Möller, Martin},
      title        = {3{D} artificial extracellular matrices for directed in
                      vitro cell growth},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2023-06997},
      pages        = {1 Online-Ressource : Illustrationen, Diagramme},
      year         = {2023},
      note         = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
                      University; Dissertation, RWTH Aachen University, 2023},
      abstract     = {This doctoral thesis reports multiple strategies to develop
                      3D aECM for directed in vitro cell growth, considering the
                      influence of different biomaterial parameters, such as
                      chemical, physical, and architectural cues. Beginning with
                      research motivation and highlighting the relevant
                      literature, the thesis probes into aECM development of
                      hierarchical scaffolds, its limitations, and the approaches
                      to specifically address these limitations. Chapter 2 mainly
                      highlights the state-of-the-art in detail starting with the
                      ECM development, engineering integrin-binding domains, and
                      fabrication techniques for surface topographies closing with
                      synthetically derived 3D scaffolds. Chapter 3 describes the
                      role of architectural cues in orienting nerve cells on 2D
                      platforms developed using a high-throughput TPL system and
                      the ability of nerves to follow discreet anisometric
                      microelements is systematically investigated for the first
                      time. Further, the role of supporting cells in creating aECM
                      platforms is realized. In Chapter 4, the need for transition
                      from 2D to 3D scaffolds is highlighted, which is essential
                      for mimicking relevant tissue models in vivo. Here,
                      synthetic PEG hydrogels tethered with novel
                      integrin-specific bicyclic RGD peptides are investigated,
                      reporting superior nerve growth compared to linear or
                      monocyclic RGD peptides. ANISOGELS made with magnetically
                      orientable fibers provided oriented nerve growth. Chapter 5
                      illustrates the importance of the viscoelastic and
                      strain-stiffening microenvironment in aECM design and the
                      role of interpenetrating networks of PIC and PEG showing
                      superior mechanical properties compared to the individual
                      gels for cell growth. Finally, chapter 6 discusses in detail
                      the techniques to spatially photo-pattern biomolecules
                      relevant for cell adhesion. Here, two different
                      photo-patterning systems are explored that do not alter the
                      biomechanical properties. The caged RGD system and the caged
                      Q/K systems both show possibilities for directed and spatial
                      control of cells. The variety of photo protective groups
                      available in different wavelengths paves the way from in
                      vitro photopatterning towards in vivo photopatterning to
                      create biochemical gradients in injectable aECM scaffold. To
                      summarize, the hydrogel reported in this thesis is a
                      versatile system that can be employed as a promising
                      injectable scaffold with integrin selective adhesion cues,
                      imparting viscoelastic properties in combination with
                      PIC-PEG IPNs, and finally enabling oriented cell growth
                      using ANISOGELS or through bio-patterning.},
      cin          = {156420 / 150000},
      ddc          = {540},
      cid          = {$I:(DE-82)156420_20190828$ / $I:(DE-82)150000_20140620$},
      pnm          = {BIOGEL - Engineering responsive and biomimetic hydrogels
                      for biomedical therapeutic and diagnostic applications
                      (642687) / ANISOGEL - Injectable anisotropic
                      microgel-in-hydrogel matrices for spinal cord repair
                      (637853) / ORGANTRANS - Controlled Organoids transplantation
                      as enabler for regenerative medicine translation (874586)},
      pid          = {G:(EU-Grant)642687 / G:(EU-Grant)637853 /
                      G:(EU-Grant)874586},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2023-06997},
      url          = {https://publications.rwth-aachen.de/record/961720},
}