% IMPORTANT: The following is UTF-8 encoded. This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.
@PHDTHESIS{Pastoors:972985,
author = {Pastoors, Johannes Tilmann},
othercontributors = {Büchs, Jochen and Palkovits, Regina},
title = {{I}ntegrating hydrophobic adsorption for $in$ $situ$
product removal into biorefinery processes},
school = {Rheinisch-Westfälische Technische Hochschule Aachen},
type = {Dissertation},
address = {Aachen},
publisher = {RWTH Aachen University},
reportid = {RWTH-2023-10514},
pages = {1 Online-Ressource : Illustrationen, Diagramme},
year = {2023},
note = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
University 2024; Dissertation, Rheinisch-Westfälische
Technische Hochschule Aachen, 2023},
abstract = {In the transition to a sustainable bioeconomy, conventional
petrochemical production routes need to be replaced by
innovative biorefinery processes. However, many of the
biotechnological alternatives are not yet economically
competitive. Areas with potential for improvement of cost
efficiency include substrate costs and downstream
processing. Consequently, the demand for novel cultivation
media and downstream concepts is growing continuously.This
thesis aimed to establish in situ adsorption on hydrophobic
adsorbents as an alternative method for product recovery for
biorefinery processes. As a defined cultivation medium is
essential for a reasonable investigation of adsorptive
processes, a minimal medium for the cultivation of
Gluconobacter oxydans was developed. Therefore, a rich
mineral medium with 48 components was systematically reduced
based on respiratory investigations in an in-house built
48-well microtitre plate-based system. The resulting minimal
medium with 17 components showed comparable results for
growth and production of 5-keto-D-fructose with G. oxydans
as the established complex medium.For a better understanding
of the interactions between adsorbents and bioprocesses, a
protocol for the investigation of bioprocess compatibility
was developed. Inhibitor release and nutrient adsorption by
a total of 13 different adsorbents was investigated for
three biological model systems. Respiratory monitoring
allowed for identifying negative influences of the treatment
with adsorbents on the bioprocess. This knowledge
facilitates the selection of a suitable adsorbent and the
compensation of nutrient adsorption by adjusting the
concentrations of the adsorbed media components.Itaconic
acid production with Usitlago cynodontis was selected as the
model process for the integration of in situ adsorption. A
sequential glucose feeding strategy was developed to keep
glucose concentrations at low values and limit the
co-adsorption of glucose. Activated carbons showed good
selectivities for the adsorption of itaconic acid from
mixtures with glucose at acidic pH values and in continuous
adsorption. As the eluent ethanol was introduced into the
fermenter during proof of concept experiments, concentration
monitoring by Raman spectroscopy was implemented for better
process control. The integrated process showed an increase
of the titre, yield and space time yield by up to $30\%$
compared to the first experiment of the study. The presented
work highlights the potential of in situ adsorption as a
part of the toolbox for designing novel biorefinery
processes.},
cin = {416510},
ddc = {620},
cid = {$I:(DE-82)416510_20140620$},
pnm = {TIB: BioSorp : Teilprojekt A; Das Potential von Adsorption
zur energieeffizienten Stofftrennung in fermentativen
Bioraffinerie-Prozessen (BMBF-031B0678A)},
pid = {G:(DE-82)BMBF-031B0678A},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2023-10514},
url = {https://publications.rwth-aachen.de/record/972985},
}
guest ::