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@PHDTHESIS{Dinger:976227,
      author       = {Dinger, Robert Martin},
      othercontributors = {Büchs, Jochen and Mayr, Torsten},
      title        = {{O}ptode-based online monitoring technologies for aerobic
                      fermentations in orbitally shaken bioreactors},
      school       = {Rheinisch-Westfälische Technische Hochschule Aachen},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {RWTH Aachen University},
      reportid     = {RWTH-2024-00089},
      pages        = {1 Online-Ressource : Illustrationen},
      year         = {2023},
      note         = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
                      University 2024; Dissertation, Rheinisch-Westfälische
                      Technische Hochschule Aachen, 2023},
      abstract     = {Two of the most essential online monitored parameters in
                      fermentations are the oxygen and the carbon dioxide transfer
                      rate (OTR and CTR). OTR and CTR-monitoring allow fast
                      characterization of bioprocesses and process transfer to
                      larger scales. Especially, 96-deepwell MTP are used for
                      high-throughput-experiments during early-stage bioprocess
                      development. However, a device for OTR and CTR monitoring in
                      96-deepwell microtiter plates (MTPs) is still not available.
                      To determine OTR and CTR, the measurement of the gas
                      composition in each well of a MTP is necessary. Therefore, a
                      new micro(μ)-scale Transfer rate Online Measurement device
                      (μTOM) was developed. Novel O2 and CO2-sensitive
                      fluorescent sensors were integrated into the μTOM-device to
                      determine the carbon dioxide transfer rate (CTR) of
                      individual wells. By including 48 CO2 and 48 O2 sensors, the
                      96-well sensor layout of the μTOM allows for the
                      calculation of 48 respiration quotients (RQs) in cultures
                      with a liquid filling volume below 1 mL. The first-ever
                      parallel monitoring of the OTR, CTR and RQ in one MTP during
                      cultivations Hansenula polymorpha and E. coli BL21(DE3) are
                      presented. The interpretation of the RQ allowed
                      distinguishing between the oxygen-unlimited growth of H.
                      polymorpha on glucose as well as the accumulation and
                      consumption of ethanol. Furthermore, the RQ was used to
                      identify the sequential consumption of glucose and glycerol
                      by E. coli BL21(DE3) in Wilms-MOPS autoinduction
                      medium.Another parameter significantly influencing culture
                      performance is the power consumption. Power consumption in
                      liquid cultivation media results in heat generation. It is
                      widely considered that the heat loss through the glass walls
                      of shake flasks is sufficient to ensure a constant
                      temperature of the liquid medium throughout cultivations.
                      This longstanding assumption was investigated using novel
                      spherical mobile sensors (Sens-o-Spheres, amensio GmbH) and
                      luminescence-based sensor spots (TPSP5, PyroScience GmbH).
                      Both sensors revealed a significant increase in fluid
                      temperature of up to 0.8 K in shaken glycerol solutions with
                      dynamic viscosities ranging from 10 to 193 mPa·s.
                      Furthermore, the metabolic activity of H. polymorpha
                      resulted in a temperature increase of 0.3 K. In conclusion,
                      the generated hydromechanical and biological heat exceeded
                      the heat loss through the glass walls.},
      cin          = {416510},
      ddc          = {620},
      cid          = {$I:(DE-82)416510_20140620$},
      pnm          = {BMBF-031B0102A - KMU-innovativ-17: µRAMOS - Entwicklung
                      einer Anlage zur Well-aufgelösten Bestimmung der
                      Atmungsaktivität von Mikroorganismen und Zellkulturen in
                      Mikrotiterplatten; TP A (-031B0102A)},
      pid          = {G:(BMBF)-031B0102A},
      typ          = {PUB:(DE-HGF)11},
      doi          = {10.18154/RWTH-2024-00089},
      url          = {https://publications.rwth-aachen.de/record/976227},
}