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TY  - THES
AU  - Schito, Simone
TI  - Community of niche-optimized strains : a novel concept for the biotechnological production of small molecules
PB  - Rheinisch-Westfälische Technische Hochschule Aachen
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2024-00279
SP  - 1 Online-Ressource : Illustrationen
PY  - 2023
N1  - Veröffentlicht auf dem Publikationsserver der RWTH Aachen University 2024
N1  - Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2023
AB  - Nowadays, most fermentation processes for the production of platform and fine chemicals utilize pure cultures of highly engineered model microorganisms with flexible metabolic capacities. However, in a bioreactor environment, these strains are characterized by high expression of unused enzymes resulting in carbon and energy waste, thereby affecting process efficiency. In contrast, microorganisms in nature have evolved in communities and are often characterized by auxotrophies. This suggests that co-cultures with leaner genomes and interactions via cross-feeding must have significant advantages over pure cultures of organisms with naturally larger genomes.To explore this hypothesis and demonstrate its potential for establishing new bioprocesses, the concept of Communities of Niche-optimized Strains (CoNoS) was developed and investigated. A model-based approach was used to create multiple CoNoS composed of co-cultures of at least two strains of the same species, each carrying a different auxotrophy. While the auxotrophies provide some carbon and energy savings due to the deletion of highly expressed genes, cross-feeding compensates for the auxotrophies and helps create a defined ecological niche in which the CoNoS can use available resources more efficiently. To establish the CoNoS, auxotrophic strains of Corynebacterium glutamicum were constructed, and rational metabolic engineering was applied to enhance amino acid exchange among community members. In this way, a fast-growing co-culture was established that exhibited a growth rate of 83
LB  - PUB:(DE-HGF)11
DO  - DOI:10.18154/RWTH-2024-00279
UR  - https://publications.rwth-aachen.de/record/976519
ER  -