h1

TY  - THES
AU  - Motta, Alessandro
TI  - 3D-printed phantoms for evaluating sensitivity and detection limits of fluorophore-labelled nanomedicines in FMT/CT imaging
PB  - Rheinisch-Westfälische Technische Hochschule Aachen
VL  - Dissertation
CY  - Aachen
M1  - RWTH-2024-01048
SP  - 100 Seiten : Illustrationen
PY  - 2024
N1  - Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2024
AB  - This thesis describes the prototyping of custom phantoms for assessing the sensitivity and detection limits of contrast agents via fluorescence reflectance imaging (FRI) and fluorescence-mediated tomography combined with micro-computed tomography (FMT/CT). The optical imaging agent employed was a nano-scale liposome formulation labeled with the near-infrared (NIR) fluorophore Cy7, with a clinically relevant composition and used at representative preclinical concentrations. Computer-aided modeling and 3D-printing techniques were employed to manufacture multi-channel phantoms with different thermoplastic materials, and subsequently, to recognize the low-end detection limit of the FMT/CT system, as well as to define its detection sensitivity by scanning various broad and narrow concentration ranges of the Cy7-nanoformulation. The multi-channel phantom facilitated the rapid acquisition and data collection in comparison to single-well phantoms. In addition, considering the different optical properties of the materials used for 3D printing, constructs composed of different materials were systematically scanned for their optical features by spectrophotometry. As a final proof of concept, mouse whole-body and key organ (heart, liver) segmentation from an in vivo dataset were averaged to extract relevant shapes and volumes, in order to generate a 3D-printed mouse-like phantom with removable and contrast agent- fillable organs, thereby allowing for simulation of fluorescence detection in organs relevant for assessing nanoparticle performance in vivo. Altogether, this thesis integrates 3D-printing approaches together with optical imaging analysis via FRI, FMT/CT and spectrophotometry for manufacturing and evaluating biomimetic phantoms towards the assessment of the sensitivity and detection limits of NIR probes and NIR-labelled nanomedicine formulations.
LB  - PUB:(DE-HGF)11
UR  - https://publications.rwth-aachen.de/record/977990
ER  -