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%0 Thesis
%A Keitel, Laura
%T Cultivation systems for anaerobic single and mixed cultures
%I Rheinisch-Westfälische Technische Hochschule Aachen
%V Dissertation
%C Aachen
%M RWTH-2024-03782
%P 1 Online-Ressource : Illustrationen
%D 2024
%Z Veröffentlicht auf dem Publikationsserver der RWTH Aachen University
%Z Dissertation, Rheinisch-Westfälische Technische Hochschule Aachen, 2024
%X The promising, yet barely investigated anaerobic strain Phocaeicola vulgatus (formerly Bacteroides vulgatus) plays a vital role for human gut health and effectively produces the industrially relevant acid succinate. Cultivating anaerobic bacteria is challenging, and a detailed understanding of P. vulgatus growth and metabolism is required to improve succinate production. Currently, P. vulgatus is mainly studied in mixed cultures, as it is its natural occurrence. In this work, axenic cultures were studied in microtiter plate, shake flask, and 2 L fermenter scales. First, the influence of initial pH, buffer concentration, osmolality, product inhibition was characterized. Second, the influence of different CO2 and O2 concentrations was tested. Third, the effect of initial glucose and NH4Cl concentration and different carbon and nitrogen sources on growth and organic acid production by P. vulgatus were examined. Cultivations were performed in an in-house built device for anaerobic online monitoring of fluorescence and scattered light (BioLector) in microtiter plate scale, a fermenter equipped with online gas monitoring and the in-house developed Respiratory Activity Monitoring System (RAMOS) in shake flask scale, which provides online monitoring of CO2, O2, and pressure under gassed conditions. HPLC analysis generated closed carbon balances in shake flask and fermenter scale, accounting for all produced acids. Total gas and CO2 transfer rates revealed that 65 
%F PUB:(DE-HGF)11
%9 Dissertation / PhD Thesis
%R 10.18154/RWTH-2024-03782
%U https://publications.rwth-aachen.de/record/983941