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@PHDTHESIS{Niehoff:984806,
author = {Niehoff, Paul-Joachim},
othercontributors = {Büchs, Jochen and Wierckx, Nick},
title = {{D}evelopment of an itaconic acid production process on
alternative feedstocks},
school = {Rheinisch-Westfälische Technische Hochschule Aachen},
type = {Dissertation},
address = {Aachen},
publisher = {RWTH Aachen University},
reportid = {RWTH-2024-04243},
pages = {1 Online-Ressource : Illustrationen},
year = {2024},
note = {Veröffentlicht auf dem Publikationsserver der RWTH Aachen
University; Dissertation, Rheinisch-Westfälische Technische
Hochschule Aachen, 2024},
abstract = {The establishment of biorefinery concepts is hindered by
their economic disadvantages compared to conventional
petrochemical production routes. For the production of bulk
chemicals via microbial cultivation, substrate costs are a
major contributor to the overall costs. Therefore, the use
of locally sourced alternative feedstocks is of key
importance for the transition towards a decentralized and
sustainable bioeconomy. In this thesis, a production process
for itaconic acid was developed using alternative feedstocks
as sole carbon source. Early-stage process development was
conducted in small-scale shaken bioreactors like microtiter
plates and shake flasks. In the first part of this work,
five different Ustilago strains were screened for their
growth and production of itaconic acid on defined media. U.
cynodontis ITA Max pH, a highly engineered production
strain, was selected to determine the biologically available
nitrogen concentration in thick juice and molasses. Based on
these findings, thick juice was chosen as feedstock to
ensure the necessary nitrogen limitation for itaconic acid
production. U. cynodontis ITA Max pH was further
characterized regarding osmotolerance and product
inhibition. A successful scale-up to a 2-liter stirred tank
reactor was accomplished without drawbacks in either titer
or yield compared to pure glucose fermentations. The process
was then further optimized regarding pH, nitrogen
concentration, and pH adjusting agent before being scaled to
a 150 L bioreactor. The fermentation shows the
proof-of-concept for the scale-up of itaconic acid
production with an alternative feedstock as the sole carbon
source. Especially early-stage bioprocess development is
impossible without shake flasks. To evaluate the suitability
of different shake flask sizes, a procedural analysis of the
commercially available shake flasks from 50 – 5000 mL was
conducted. This analysis showed that no general
recommendation for a specific parameter set can be made,
which is valid for all cultivations. These rather depend on
the chosen production strain and process. This study
describes an approach for fast and reliable process
development for biorefinery concepts and highlights the
importance of engineering parameters for the successful
implementation of shake flasks within the process
development of these concepts.},
cin = {416510},
ddc = {620},
cid = {$I:(DE-82)416510_20140620$},
pnm = {BMBF 031B0918E - $BioökonomieREVIER_INNO:$ Entwicklung der
Modellregion BioökonomieREVIER Rheinland, TP E: Upcycling
organischer Reststoffe für die chemische Industrie (UpRePP)
(BMBF-031B0918E)},
pid = {G:(DE-82)BMBF-031B0918E},
typ = {PUB:(DE-HGF)11},
doi = {10.18154/RWTH-2024-04243},
url = {https://publications.rwth-aachen.de/record/984806},
}
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