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@PHDTHESIS{Grtering:987836,
      author       = {Grütering, Carolin},
      othercontributors = {Blank, Lars M. and Jupke, Andreas},
      title        = {{P}roducing methyl ketones in integrated bioprocesses; 1.
                      {A}uflage},
      volume       = {34},
      school       = {RWTH Aachen University},
      type         = {Dissertation},
      address      = {Aachen},
      publisher    = {Apprimus Verlag},
      reportid     = {RWTH-2024-05867},
      isbn         = {978-3-98555-221-4},
      series       = {Applied microbiology},
      pages        = {1 Online-Ressource : Illustrationen},
      year         = {2024},
      note         = {Druckausgabe: 2024. - Auch veröffentlicht auf dem
                      Publikationsserver der RWTH Aachen University; Dissertation,
                      RWTH Aachen University, 2024},
      abstract     = {Short- and medium-chain length methyl ketones such as
                      2-butanone and 2-undecanone are important commodity
                      chemicals that are currently produced from petrochemical
                      resources. To date, there is only limited research regarding
                      the biotechnological production and purification of those
                      methyl ketones in the scope of acircular bioeconomy. This
                      work elucidates integrated bioprocesses for the production
                      of methyl ketones using genetically modified microorganisms.
                      Methyl ketones with a chain length of C11 to C17 are
                      produced by genetically modified Pseudomonas taiwanensis
                      VLB120 in a bioprocess with addition of an organic solvent
                      to the cultivation medium for in situ liquid-liquid product
                      extraction. The applied organicsolvent can decisively
                      influence important process parameters. However, the type
                      ofsolvent for in situ extraction of methyl ketones was not
                      investigated so far, and one ofthe major challenges of in
                      situ liquid-liquid extraction, the formation of stable
                      emulsions, is still unsolved. This work describes an
                      in-depth investigation of theorganic solvent for in situ
                      product extraction of methyl ketones and the subsequent
                      recovery of the organic phase. By performing a hierarchical
                      solvent screening, wefound 2-undecanone as a solvent that is
                      biocompatible, non-biodegradable, and safe.With 2-undecanone
                      as a solvent in an advanced bioreactor setup, the multiphase
                      loopreactor, formation of stable emulsions was successfully
                      circumvented. Here,countercurrent liquid-liquid extraction
                      occurred in a downcomer compartment, and theorganic phase
                      could be recovered by decantation in a coalescing unit.
                      Subsequentexaminations highlighted the methyl ketone blend's
                      potential as a drop-in diesel fuel replacement.
                      Investigations in, e.g., a single-cylinder research engine
                      demonstrated efficient and clean combustion with little NOx
                      and soot emissions.As a next step, the carbon sources that
                      are converted to the C11 to C17 methyl ketonesand the
                      associated feeding strategies were investigated. By an in
                      silico screening approach using a genome-scale metabolic
                      model of the production host, ethanol wasfound as a co-feed
                      carbon source that enabled superior product yields.
                      Additionally, methyl ketone production was shown to be
                      possible by utilizing lignocellulosichydrolysates. Notably,
                      also the hemicellulosic fraction that contains xylose as a
                      carbonsource and inhibitors such as furfural and vanillin
                      was converted to methyl ketones. A bioprocess cascade was
                      also developed for the production of C4 methyl
                      ketonesacetoin and 2-butanone. Acetoin was produced by
                      resting cells of Lactococcus lactisat product yields close
                      to the the oretical maximum. The resting cell buffer was
                      tailored to meet the requirements of an ensuing
                      electrocatalytic reduction, transforming biotechnologically
                      produced acetoin into 2-butanone. Concluding, the
                      bioprocesses for the biotechnological production of methyl
                      ketoneswere developed and improved in a holistic manner.
                      Biotechnological methods were integrated into the preceding
                      and consecutive process steps and optimized beyond their
                      isolated figures of merit.},
      cin          = {161710 / 160000},
      ddc          = {570},
      cid          = {$I:(DE-82)161710_20140620$ / $I:(DE-82)160000_20140620$},
      pnm          = {DFG project 390919832 - EXC 2186: Das Fuel Science Center
                      – Adaptive Umwandlungssysteme für erneuerbare Energie-
                      und Kohlenstoffquellen (390919832) / BioSC - Bioeconomy
                      Science Center (BioSC)},
      pid          = {G:(GEPRIS)390919832 / G:(DE-Juel1)BioSC},
      typ          = {PUB:(DE-HGF)11 / PUB:(DE-HGF)3},
      doi          = {10.18154/RWTH-2024-05867},
      url          = {https://publications.rwth-aachen.de/record/987836},
}